'PerlPrimer' designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR), and sequencing by automating and simplifying the process of primer design. Current features include calculation of possible primer-dimers, retrieval of genomic or CDNA sequences from Ensembl, the ability to BLAST search primers using the NCBI server, ORF, and CpG island detection algorithms, the ability to add cloning sequences to primers, automatically adjusted to be in-frame, and QPCR primer design without manual intron-exon boundary entry.
DocumentationUser tutorial available in HTML format from http://perlprimer.sourceforge.net/tutorial.html
released on 11 August 2005
|License||Verified by||Verified on||Notes|
|GPLv2orlater||Janet Casey||21 June 2004|
Leaders and contributors
Resources and communication
|Required to use||Perl/Tk|
|Required to use||Perl|
|Required to use||libwww-perl 5.76 or later|
This entry (in part or in whole) was last reviewed on 7 January 2008.
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