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'PerlPrimer' designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR), and sequencing by automating and simplifying the process of primer design. Current features include calculation of possible primer-dimers, retrieval of genomic or CDNA sequences from Ensembl, the ability to BLAST search primers using the NCBI server, ORF, and CpG island detection algorithms, the ability to add cloning sequences to primers, automatically adjusted to be in-frame, and QPCR primer design without manual intron-exon boundary entry.


User tutorial available in HTML format from


Download version 1.1.8 (stable)
released on 11 August 2005



LicenseVerified byVerified onNotes
GPLv2orlaterJanet Casey21 June 2004

Leaders and contributors

Owen Marshall Maintainer

Resources and communication

Audience Resource type URI
Bug Tracking,Developer,Support E-mail

Software prerequisites

Kind Description
Required to use libwww-perl 5.76 or later
Required to use Perl/Tk
Required to use Perl

This entry (in part or in whole) was last reviewed on 7 January 2008.


Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.3 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the page “GNU Free Documentation License”.

The copyright and license notices on this page only apply to the text on this page. Any software or copyright-licenses or other similar notices described in this text has its own copyright notice and license, which can usually be found in the distribution or license text itself.

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