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Designs primers for standard, bisulphite, and real-time PCR, and sequencing

'PerlPrimer' designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR), and sequencing by automating and simplifying the process of primer design. Current features include calculation of possible primer-dimers, retrieval of genomic or CDNA sequences from Ensembl, the ability to BLAST search primers using the NCBI server, ORF, and CpG island detection algorithms, the ability to add cloning sequences to primers, automatically adjusted to be in-frame, and QPCR primer design without manual intron-exon boundary entry.



Verified by

Verified on


Verified by

Janet Casey

Verified on

21 June 2004

Leaders and contributors

Owen Marshall Maintainer

Resources and communication

AudienceResource typeURI
Bug Tracking,Developer,

Software prerequisites

Required to usePerl/Tk
Required to usePerl
Required to uselibwww-perl 5.76 or later


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